Guidelines for the Testing of Chemicals
Section 1: Physical Chemical Properties
| OECD Guidelines for the Testing of Chemicals | MEE Guidelines for the Testing of Chemicals | National standard (GB) |
| Test No. 101: UV-VIS Absorption Spectra | 101 UV-VIS Absorption Spectra — Spectrophotometric Method | |
| Test No. 102: Melting Point/ Melting Range | 102 Melting Point/ Melting Range | |
| Test No. 103: Boiling Point | 103 Boiling Point | |
| Test No. 104: Vapour Pressure | 104 Vapour Pressure | |
| Test No. 105: Water Solubility | 105 Water Solubility | GB/T 21845-2008 Chemicals – Water solubility test |
| Test No. 106: Adsorption — Desorption Using a Batch Equilibrium Method | 106 Adsorption-Desorption Using a Batch Equilibrium Method | GB/T 21851-2008 Chemicals – Adsorption – desorption using a batch equilibrium method |
| Test No. 107: Partition Coefficient (n-octanol/water): Shake Flask Method | 107 Partition Coefficient (n-octanol/water): Shake Flask Method | GB/T 21853-2008 Testing of chemicals – Partition coefficient（n-octanol/water）- Shake flask method |
| Test No. 108: Complex Formation Ability in Water | 108 Complex Formation Ability in Water — Polarographic Method | |
| Test No. 109: Density of Liquids and Solids | 109 Density of Liquids and Solids | |
| Test No. 110: Particle Size Distribution/ Fibre Length and Diameter Distributions | 110 Particle Size Distribution/ Fibre Length and Diameter Distributions
Method A: Particle Size Distribution (effective hydrodynamic radius) Method B: Fibre Length and Diameter Distributions |
GB/T 22231-2008 Particle size distribution/fibre length and diameter distributions |
| Test No. 111: Hydrolysis as a Function of pH | 111 Hydrolysis as a Function of pH | GB/T 21855-2008 Chemicals – Test of hydrolysis as a function of pH |
| Test No. 112: Dissociation Constants in Water | 112 Dissociation Constants in Water (Titration Method-Spectrophotometric Method-Conductometric Method) | |
| Test No. 113: Screening Test for Thermal Stability and Stability in Air | 113 Screening Test for Thermal Stability and Stability in Air
Accelerated Storage Test [CIPAC-Test]-Thermal Analysis Methods, including differential thermal analysis [DATA] and thermogravimetric analysis [TGA] |
GB/T 22232-2008 Test method for the thermal stability of chemicals by differential scanning calorimetry |
| Test No. 114: Viscosity of Liquids | 114 Viscosity of Liquids | GB/T 27846-2011 Chemicals – Measurement of viscosity using the Hoppler falling-ball viscometer |
| Test No. 115: Surface Tension of Aqueous Solutions | 115 Surface Tension of Aqueous Solutions | GB/T 27842-2011 Chemicals – Test method for dynamic surface tension – Fast-bubble technique |
| Test No. 116: Fat Solubility of Solid and Liquid Substances | 116 Fat Solubility of Solid and Liquid Substances — Flask Method | |
| Test No. 117: Partition Coefficient (n-octanol/water), HPLC Method | 117 Partition Coefficient (n-octanol/water) — High performance liquid chromatography (HPLC) method | GB/T 21852-2008 Testing of chemicals – Partition coefficient（n-octanol/water）- High performance liquid chromatography（HPLC）method |
| Test No. 118: Determination of the Number-Average Molecular Weight and the Molecular Weight Distribution of Polymers using Gel Permeation Chromatography | 118 Determination of the Number-Average Molecular Weight and the Molecular Weight Distribution of Polymers using Gel Permeation Chromatography | |
| Test No. 119: Determination of the Low Molecular Weight Content of a Polymer Using Gel Permeation Chromatography | 119 Determination of the Low Molecular Weight Content of a Polymer Using Gel Permeation Chromatography | GB/T 27843-2011 Chemicals – Determination of the low molecular weight content of a polymer using Gel permeation chromatography（GPC） |
| Test No. 120: Solution/Extraction Behaviour of Polymers in Water | 120 Solution/Extraction Behaviour of Polymers in Water | |
| Test No. 121: Estimation of the Adsorption Coefficient (Koc ) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC) | 121 Estimation of the Adsorption Coefficient (Koc ) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC) | GB/T 27860-2011 Chemicals – Estimation of the adsorption coefficient (Koc) on soil and on sewage sludge using high performance liquid chromatography (HPLC) |
| Test No. 122: Determination of pH, Acidity and Alkalinity | ||
| Test No. 123: Partition Coefficient (1-Octanol/Water): Slow-Stirring Method | 123 Partition Coefficient (1-Octanol/Water): Slow-Stirring Method | GB/T 29882-2013 Testing method for classification of hazardous materials – Partition coefficient of 1-octanol/water |
| Test No. 124: Determination of the Volume Specific Surface Area of Manufactured Nanomaterials | ||
| Test No. 125: Nanomaterial Particle Size and Size Distribution of Nanomaterials | ||
| 191 pH value |
Section 2: Effects on Biotic Systems
| OECD Guidelines for the Testing of Chemicals | MEE Guidelines for the Testing of Chemicals | National standard (GB) |
| Test No. 201: Freshwater Alga and Cyanobacteria, Growth Inhibition Test | 201 Alga Growth Inhibition Test | GB/T 21805-2008 Testing of chemicals – Alga growth inhibition test |
| Test No. 202: Daphnia sp. Acute Immobilisation Test | 202 Daphnia sp. EC50 Acute Immobilisation Test | GB/T 21830-2008 Testing of chemicals – Daphnia sp., acute immobilisation test |
| Test No. 203: Fish, Acute Toxicity Test | 203 Fish Acute Toxicity Test | GB/T 27861-2011 Chemicals – Fish acute toxicity test |
| Test No. 204: Fish, Prolonged Toxicity Test: 14-Day Study | 204 Fish, Prolonged Toxicity Test: 14-Day Study | |
| Test No. 205: Avian Dietary Toxicity Test | 205 Avian Dietary Toxicity Test | GB/T 21810-2008 Chemicals – Avian dietary toxicity test |
| Test No. 206: Avian Reproduction Test | 206 Avian Reproduction Test | GB/T 21811-2008 Chemicals – Avian reproduction test |
| Test No. 207: Earthworm, Acute Toxicity Tests | 207 Earthworm, Acute Toxicity Tests | GB/T 21809-2008 Chemicals – Test method of earthworm, acute toxicity test |
| Test No. 208: Terrestrial Plant Test: Seedling Emergence and Seedling Growth Test | 208 Terrestrial Plants, Growth Test | |
| Test No. 209: Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation) | 209 Activated Sludge, Respiration Inhibition Test | GB/T 21796-2022Chemicals—Activated sludge respiration inhibition test |
| Test No. 210: Fish, Early-life Stage Toxicity Test | 210 Fish, Early-life Stage Toxicity Test | GB/T 21854-2008 Testing of chemicals – Fish, early-life stage toxicity test |
| Test No. 211: Daphnia magna Reproduction Test | 211 Daphnia magna Reproduction Test | GB/T 21828-2008 Testing of chemicals – Daphnia magna reproduction test |
| Test No. 212: Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages | 212 Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages | GB/T 21807-2008 Testing of chemicals – Fish, short-term toxicity test on embryo and sac-fry stages |
| Test No. 213: Honeybees, Acute Oral Toxicity Test | 213 Honeybees, Acute Oral Toxicity Test | GB/T 21812-2008 Chemicals – Honeybees, acute oral toxicity test |
| Test No. 214: Honeybees, Acute Contact Toxicity Test | 214 Honeybees, Acute Contact Toxicity Test | GB/T 21813-2008 Chemicals – Honeybees acute contact toxicity test |
| Test No. 215: Fish, Juvenile Growth Test | 215 Fish, Juvenile Growth Test | GB/T 21806-2008 Testing of chemicals – Fish, juvenile growth test |
| Test No. 216: Soil Microorganisms: Nitrogen Transformation Test | 216 Soil Microorganisms: Nitrogen Transformation Test | GB/T 27854-2011 Chemicals – Soil microorganisms – Nitrogen transformation test |
| Test No. 217: Soil Microorganisms: Carbon Transformation Test | 217 Soil Microorganisms: Carbon Transformation Test | GB/T 27855-2011 Chemicals – Soil microorganisms – Carbon transformation test |
| Test No. 218: Sediment-Water Chironomid Toxicity Using Spiked Sediment | 218 Sediment-Water Chironomid Toxicity Using Spiked Sediment | GB/T 27859-2011 Chemicals – Sediment-water chironomid toxicity test – Spiked sediment method |
| Test No. 219: Sediment-Water Chironomid Toxicity Using Spiked Water | 219 Sediment-Water Chironomid Toxicity Using Spiked Water | GB/T 27858-2011 Chemicals – Sediment-water chironomid toxicity test – Spiked water method |
| Test No. 220: Enchytraeid Reproduction Test | 220 Enchytraeid Reproduction Test | GB/T 35514-2017 Chemicals—Enchytraeid reproduction test |
| Test No. 221: Lemna sp. Growth Inhibition Test | 221 Lemna sp. Growth Inhibition Test | GB/T 35524-2017 Chemicals—Lemna sp. growth inhibition test |
| Test No. 222: Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei) | 222 Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei) | |
| Test No. 223: Avian Acute Oral Toxicity Test | 223 Avian Acute Oral Toxicity Test | GB/T 41623-2022 Chemicals—Avian acute oral toxicity test |
| Test No. 224: Determination of the Inhibition of the Activity of Anaerobic Bacteria | 224 Anaerobic Biodegradability of Organic Compounds in Digested Sludge: By Measurement of Gas Production | |
| Test No. 225: Sediment-Water Lumbriculus Toxicity Test Using Spiked Sediment | 225 Sediment-Water Lumbriculus Toxicity Test Using Spiked Sediment | |
| Test No. 226: Predatory mite (Hypoaspis (Geolaelaps) aculeifer) reproduction test in soil | 226 Predatory Mite [Hypoaspis (Geolaelaps) aculeifer] Reproduction Test in Soil | |
| Test No. 227: Terrestrial Plant Test: Vegetative Vigour Test | 227 Terrestrial Plant Test: Vegetative Vigour Test | GB/T 27851-2011 Chemicals – Terrestrial plant test – Vegetative vigour test |
| Test No. 228: Determination of Developmental Toxicity to Dipteran Dung Flies (Scathophaga stercoraria L. (Scathophagidae), Musca autumnalis De Geer (Muscidae)) | 228 Determination of Developmental Toxicity of a Test Chemical to Dipteran Dung Flies [Scathophaga stercoraria L. (Scathophagidae), Musca autumnalis De Geer (Muscidae)] Test | |
| Test No. 229: Fish Short Term Reproduction Assay | 229 Fish Short Term Reproduction Assay | |
| Test No. 230: 21-day Fish Assay | 23021-day Fish Assay: A Short-Term Screening for Oestrogenic and Androgenic Activity, and Aromatase Inhibition | |
| Test No. 231: Amphibian Metamorphosis Assay | 231 The Amphibian Metamorphosis Assay | GB/T 30664-2014 Chemicals―Amphibian metamorphosis assay |
| Test No. 232: Collembolan Reproduction Test in Soil | 232 Collembolan Reproduction Test in Soil | GB/T 35522-2017 Chemicals—Collembolan reproduction test in soil |
| Test No. 233: Sediment-Water Chironomid Life-Cycle Toxicity Test Using Spiked Water or Spiked Sediment | 233 Sediment-Water Chironomid Life-Cycle Toxicity Test — Using Spiked Water or Spiked Sediment | |
| Test No. 234: Fish Sexual Development Test | 234 Fish Sexual Development Test | |
| Test No. 235: Chironomus sp., Acute Immobilisation Test | 235 Chironomus sp., Acute Immobilisation Test | |
| Test No. 236: Fish Embryo Acute Toxicity (FET) Test | ||
| Test No. 237: Honey Bee (Apis Mellifera) Larval Toxicity Test, Single Exposure | ||
| Test No. 238: Sediment-Free Myriophyllum Spicatum Toxicity Test | ||
| Test No. 239: Water-Sediment Myriophyllum Spicatum Toxicity Test | ||
| Test No. 240: Medaka Extended One Generation Reproduction Test (MEOGRT) | ||
| Test No. 241: The Larval Amphibian Growth and Development Assay (LAGDA) | ||
| Test No. 242: Potamopyrgus antipodarum Reproduction Test | ||
| Test No. 243: Lymnaea stagnalis Reproduction Test | ||
| Test No. 244: Protozoan Activated Sludge Inhibition Test | ||
| Test No. 245: Honey Bee (Apis Mellifera L.), Chronic Oral Toxicity Test (10-Day Feeding) | ||
| Test No. 246: Bumblebee, Acute Contact Toxicity Test | ||
| Test No. 247: Bumblebee, Acute Oral Toxicity Test | ||
| Test No. 248: Xenopus Eleutheroembryonic Thyroid Assay (XETA) | ||
| Test No. 249: Fish Cell Line Acute Toxicity – The RTgill-W1 cell line assay | ||
| Test No. 250: EASZY assay – Detection of Endocrine Active Substances, acting through estrogen receptors, using transgenic tg(cyp19a1b:GFP) Zebrafish embrYos | ||
| Test No. 251: Rapid Androgen Disruption Activity Reporter (RADAR) assay | ||
| 299 Seed Germination/Root Elongation Toxicity Test |
Section 3: Environmental Fate and Behaviour
| OECD Guidelines for the Testing of Chemicals | MEE Guidelines for the Testing of Chemicals | National standard (GB) |
| Test No. 301: Ready Biodegradability | 301 Ready Biodegradability | GB/T 27850-2011 Chemicals－Ready biodegradability－General considerations |
| 301A DOC Die-Away Test | 301A DOC Die-Away Test | GB/T 21803-2008 Testing of chemicals – Ready biodegradability – DOC die-away test |
| 301B CO2 Evolution Test | 301B CO2 Evolution Test | GB/T 21856-2008 Testing of chemicals – Ready biodegradability – CO2 evolution test |
| 301C Modified MITI Test (I) | 301C Modified MITI Test (I) | GB/T 21802-2008 Testing of chemicals – Ready biodegradability – Modified MITI test （I） |
| 301D Closed Bottle Test | 301D Closed Bottle Test | GB/T 21831-2008 Testing of chemicals – Ready biodegradability – Closed bottle test |
| 301E Modified OECD Screening Test | 301E Modified OECD Screening Test | GB/T 21857-2008 Testing of chemicals – Ready biodegradability – Modified OECD screening test |
| 301F Manometric Respirometry Test | 301F Manometric Respirometry Test | GB/T 21801-2008 Testing of chemicals – Ready biodegradability – Manometric respirometry test |
| Test No. 302A: Inherent Biodegradability: Modified SCAS Test | 302A Inherent Biodegradability: Modified SCAS Test | GB/T 21817-2008 Testing of chemicals – Inherent biodegradability – Modified SCAS test |
| Test No. 302B: Inherent Biodegradability: Zahn-Wellens/ EVPA Test | 302B Inherent Biodegradability: Zahn-Wellens/ EVPA Test | GB/T 21816-2008 Testing of chemicals – Inherent biodegradability – Zahn-Wellens test |
| Test No. 302C: Inherent Biodegradability: Modified MITI Test (II) | 302C Inherent Biodegradability: Modified MITI Test (II) | GB/T 21818-2008 Testing of chemicals – Inherent biodegradability – Modified MITI test （II） |
| Test No. 303: Simulation Test – Aerobic Sewage Treatment — A: Activated Sludge Units; B: Biofilms | 303A Simulation Test – Aerobic Sewage Treatment: Activated Sludge Units | GB/T 21829-2008 Testing of chemicals – Simulation test Aerobic sewage treatment – Aactivated sludge units |
| 303B Simulation Test – Aerobic Sewage Treatment: Biofilms | GB/T 21795-2008 Testing of chemicals – Simulation test Aerobic sewage treatment – Biofilms | |
| Test No. 304A: Inherent Biodegradability in Soil | 304A Inherent Biodegradability in Soil | GB/T 35518-2017 Chemicals—Inherent biodegradability in soil test |
| Test No. 305: Bioaccumulation in Fish: Aqueous and Dietary Exposure | 305 Bioconcentration – Flow-through Fish Test | GB/T 21800-2008 Testing of chemicals Bioconcentration – Flow-through fish test |
| 305A Bioconcentration – Sequential Static Fish Test | ||
| 305B Bioconcentration – Semi-Static Fish Test | GB/T 21858-2008 Testing of chemicals – Bioconcentration – Semi-static fish test | |
| 305C Bioconcentration – Test for the Degree of Bioconcentration in Fish | ||
| 305D Bioconcentration – Static Fish Test | ||
| Test No. 306: Biodegradability in Seawater | 306 Biodegradability in Seawater | |
| 306A Biodegradability in Seawater — Shake Flask Method | GB/T 21815.1-2008 Testing of chemicals Biodegradability in seawater – Shake flask method | |
| 306B Biodegradability in Seawater — Closed Bottle Method | GB/T 30665-2014 Chemicals―Biodegradability in seawater―Closed bottle method | |
| Test No. 307: Aerobic and Anaerobic Transformation in Soil | 307 Aerobic and Anaerobic Transformation in Soil | GB/T 27856-2011 Chemicals – Aerobic and anaerobic transformation in soil test |
| Test No. 308: Aerobic and Anaerobic Transformation in Aquatic Sediment Systems | 308 Aerobic and Anaerobic Transformation in Aquatic Sediment Systems | GB/T 27853-2011 Chemicals – Aerobic and anaerobic transformation in aquatic sediment systems test |
| Test No. 309: Aerobic Mineralisation in Surface Water – Simulation Biodegradation Test | 309 Aerobic Mineralisation in Surface Water – Simulation Biodegradation Test | GB/T 35523-2017 Chemicals—Aerobic mineralisation in surface water—Simulation biodegradation test |
| Test No. 310: Ready Biodegradability – CO2 in sealed vessels (Headspace Test) | 310 Ready Biodegradability – CO2 in sealed vessels (Headspace Test) | |
| Test No. 311: Anaerobic Biodegradability of Organic Compounds in Digested Sludge: by Measurement of Gas Production | 311 Anaerobic Biodegradability of Organic Compounds in Digested Sludge — By Measurement of Gas Production | GB/T 27857-2011 Chemicals – Anaerobic biodegradability of organic compounds in digested sludge – By measurement of gas production |
| Test No. 312: Leaching in Soil Columns | 312 Leaching in Soil Columns | GB/T 41667-2022 Chemicals—Testing of leaching in soil columns |
| Test No. 313: Estimation of Emissions from Preservative – Treated Wood to the Environment | 313 Estimation of Emissions from Preservative – Treated Wood to the Environment – Laboratory Method for Wooden Commodities that are not Covered and are in Contact with Fresh Water or Seawater | GB/T 41668-2022 Chemicals—Estimation of emissions from preservative-treated wood to the environment |
| Test No. 314: Simulation Tests to Assess the Biodegradability of Chemicals Discharged in Wastewater | 314 Simulation Tests to Assess the Biodegradability of Chemicals Discharged in Wastewater | |
| 314A Biodegradation in a Sewer System | ||
| 314B Biodegradation in Activated Sludge | ||
| 314C Mineralization and Transformation in Anaerobic Digester Sludge | ||
| 314D Biodegradation in Treated Effluent-surface Water Mixing Zone | ||
| 314E Biodegradation in Untreated Wastewater-surface Water Mixing Zone | ||
| Test No. 315: Bioaccumulation in Sediment-dwelling Benthic Oligochaetes | 315 Bioaccumulation in Sediment-dwelling Benthic Oligochaetes | GB/T 35527-2017 Chemicals—Bioaccumulation in sediment-dwelling benthic oligochaetes |
| Test No. 316: Phototransformation of Chemicals in Water – Direct Photolysis | 316 Phototransformation of Chemicals in Water – Direct Photolysis | |
| Test No. 317: Bioaccumulation in Terrestrial Oligochaetes | 317 Bioaccumulation in Terrestrial Oligochaetes | |
| Test No. 318: Dispersion Stability of Nanomaterials in Simulated Environmental Media | ||
| Test No. 319A: Determination of in vitro intrinsic clearance using cryopreserved rainbow trout hepatocytes (RT-HEP) | ||
| Test No. 319B: Determination of in vitro intrinsic clearance using rainbow trout liver S9 sub-cellular fraction (RT-S9) | ||
| Test No. 320: Determining Anaerobic Transformation of Chemicals in Liquid Manure | ||
| 399 Update and Accumulation Test |
Section 4: Health Effects
| OECD Guidelines for the Testing of Chemicals | MEE Guidelines for the Testing of Chemicals | National standard (GB) |
| Test No. 401: Acute Oral Toxicity | 401 Acute Oral Toxicity | GB/T 21603-2008 Test method of acute oral toxicity for chemicals |
| Test No. 402: Acute Dermal Toxicity | 402 Acute Dermal Toxicity | GB/T 21606-2022 Chemicals—Test method of acute dermal toxicity |
| Test No. 403: Acute Inhalation Toxicity | 403 Acute Inhalation Toxicity | GB/T 21605-2008 Test method of acute inhalation toxicity for chemicals |
| Test No. 404: Acute Dermal Irritation/Corrosion | 404 Acute Dermal Irritation/Corrosion | GB/T 21604-2022 Chemicals—Test method of acute dermal irritation/corrosion |
| Test No. 405: Acute Eye Irritation/Corrosion | 405 Acute Eye Irritation/Corrosion | GB/T 21609-2008 Test method of acute dermal irritation/corrosion for chemicals |
| Test No. 406: Skin Sensitisation | 406 Skin Sensitisation | GB/T 21608-2008 Test method of skin sensitization for chemicals |
| Test No. 407: Repeated Dose 28-day Oral Toxicity Study in Rodents | 407 Repeated Dose 28-day Oral Toxicity Study in Rodents | GB/T 21752-2008 Chemicals – Test method of repeated dose 28-day oral toxicity study in rodents |
| Test No. 408: Repeated Dose 90-Day Oral Toxicity Study in Rodents | 408 Repeated Dose 90-Day Oral Toxicity Study in Rodents | GB/T 21763-2008 Chemicals – Test method of repeated dose oral toxicity study in rodents |
| Test No. 409: Repeated Dose 90-Day Oral Toxicity Study in Non-Rodents | 409 Repeated Dose 90-Day Oral Toxicity Study in Non-Rodents | GB/T 21778-2008 Chemicals – Test method of repeated dose 90-day oral toxicity in non-rodents |
| Test No. 410: Repeated Dose Dermal Toxicity: 21/28-day Study | 410 Repeated Dose Dermal Toxicity: 21/28-day Study | GB/T 21753-2008 Chemicals – Test method of repeated dose dermal toxicity:21/28-day study |
| Test No. 411: Subchronic Dermal Toxicity: 90-day Study | 411 Subchronic Dermal Toxicity: 90-day Study | GB/T 21764-2008 Chemicals – Test method of subchronic dermal toxicity study |
| Test No. 412: Subacute Inhalation Toxicity: 28-Day Study | 412 Subacute Inhalation Toxicity: 28-Day Study | GB/T 21754-2008 Chemicals – Test method of repeated dose inhalation toxicity:28/14-day study |
| Test No. 413: Subchronic Inhalation Toxicity: 90-day Study | 413 Subchronic Inhalation Toxicity: 90-day Study | GB/T 21765-2008 Chemicals – Test method of subchronic inhalation toxicity study |
| Test No. 414: Prenatal Developmental Toxicity Study | 414 Prenatal Developmental Toxicity Study | |
| Test No. 415: One-Generation Reproduction Toxicity Study | 415 One-Generation Reproduction Toxicity Study | GB/T 21607-2022 Chemicals—Test method of one-generation reproduction toxicity |
| Test No. 416: Two-Generation Reproduction Toxicity | 416 Two-Generation Reproduction Toxicity | GB/T 21758-2008 Chemicals – Test method of two-generation reproduction toxicity study |
| Test No. 417: Toxicokinetics | 417 Toxicokinetics | GB/T 21750-2008 Chemicals – Test method of toxicokinetics studies |
| Test No. 418: Delayed Neurotoxicity of Organophosphorus Substances Following Acute Exposure | 418 Delayed Neurotoxicity of Organophosphorus Substances Following Acute Exposure | GB/T 21770-2008 Chemicals(organophosphorus substances) – Test method of delayed neurotoxicity following acute exposure |
| Test No. 419: Delayed Neurotoxicity of Organophosphorus Substances: 28-day Repeated Dose Study | 419 Delayed Neurotoxicity of Organophosphorus Substances: 28-day Repeated Dose Study | GB/T 21797-2008 Testing of chemicals – Delayed neurotoxicity of organophosphorus substances 28-day repeated dose study |
| Test No. 420: Acute Oral Toxicity – Fixed Dose Procedure | 420 Acute Oral Toxicity – Fixed Dose Procedure | |
| Test No. 421: Reproduction/Developmental Toxicity Screening Test | 421 Reproduction/Developmental Toxicity Screening Test | GB/T 21766-2008 Chemicals – Test method of reproduction/developmental toxicity screening |
| Test No. 422: Combined Repeated Dose Toxicity Study with the Reproduction/Developmental Toxicity Screening Test | 422 Combined Repeated Dose Toxicity Study with the Reproduction/Developmental Toxicity Screening Test | GB/T 21771-2008 Chemicals – Test method of combined repeated dose toxicity study with the reproduction/developmental toxicity screening |
| Test No. 423: Acute Oral toxicity – Acute Toxic Class Method | 423 Acute Oral toxicity – Acute Toxic Class Method | |
| Test No. 424: Neurotoxicity Study in Rodents | 424 Neurotoxicity Study in Rodents | GB/T 21787-2008 Chemicals – Test method of neurotoxicity study in rodents |
| Test No. 425: Acute Oral Toxicity: Up-and-Down Procedure | 425 Acute Oral Toxicity: Up-and-Down Procedure | GB/T 21826-2008 Chemicals – Test method of acute oral toxicity – Up and down procedure (UDP) |
| Test No. 426: Developmental Neurotoxicity Study | 426 Developmental Neurotoxicity Study | GB/T 27826-2011 Chemicals – Test method of developmental neurotoxicity |
| Test No. 427: Skin Absorption: In Vivo Method | 427 Skin Absorption: In Vivo Method | GB/T 27825-2011 Chemicals – Test method for skin absorption – In vivo |
| Test No. 428: Skin Absorption: In Vitro Method | 428 Skin Absorption: In Vivo Method | GB/T 27818-2011 Chemicals – Testing method for skin absorption-in vitro |
| Test No. 429: Skin Sensitisation | 429 Skin Sensitisation | GB/T 21827-2008 Chemicals – Test method of skin sensitization – Local lymph node assay （LLNA） |
| Test No. 430: In Vitro Skin Corrosion: Transcutaneous Electrical Resistance Test Method (TER) | 430 In Vitro Skin Corrosion: Transcutaneous Electrical Resistance Test Method (TER) | GB/T 27828-2011 Chemicals – Test method of in vitro skin corrosion – Transcutaneous electrical resistance test（TER） |
| Test No. 431: In vitro skin corrosion: reconstructed human epidermis (RHE) test method | 431 In vitro skin corrosion: reconstructed human epidermis (RHE) test method | GB/T 27830-2011 Chemicals – Test method of in vitro skin corrosion – Human skin model test |
| Test No. 432: In Vitro 3T3 NRU Phototoxicity Test | 432 In Vitro 3T3 NRU Phototoxicity Test | GB/T 21769-2008 Chemical – In vitro 3T3 NRU phototoxicity test method |
| Test No. 433: Acute Inhalation Toxicity: Fixed Concentration Procedure | ||
| Test No. 435: In Vitro Membrane Barrier Test Method for Skin Corrosion | 435 In Vitro Membrane Barrier Test Method for Skin Corrosion | GB/T 27829-2011 Chemicals – In vitro test of skin corrosion – Membrane barrier method |
| Test No. 436: Acute Inhalation Toxicity – Acute Toxic Class Method | 436 Acute Inhalation Toxicity – Acute Toxic Class Method | GB/T 28648-2012 Chemicals – Acute inhalation toxicity testing – Acute toxic class method |
| Test No. 437: Bovine Corneal Opacity and Permeability Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage | 437 Bovine Corneal Opacity and Permeability Test, BCOP | |
| Test No. 438: Isolated Chicken Eye Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage | 438 Isolated Chicken Eye Test, ICE | |
| Test No. 439: In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method | 439 In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method | |
| Test No. 440: Uterotrophic Bioassay in Rodents | 440 Uterotrophic Bioassay in Rodents: A short-term Screening Test for Oestrogenic Properties | |
| Test No. 441: Hershberger Bioassay in Rats | 441 Hershberger Bioassay in Rats: A Short-term Screening Assay for (Anti) Androgenic Properties | GB/T 35526-2017 Chemicals—A short-term screening assay for (anti) androgenic properties—Hershberger bioassay in rats |
| Test No. 442A: Skin Sensitization | 442A Local Lymph Node Assay — DA | |
| Test No. 442B: Skin Sensitization | 442B Local Lymph Node Assay — BrdU-ELISA | |
| Test No. 442C: In Chemico Skin Sensitisation | ||
| Test No. 442D: In Vitro Skin Sensitisation | ||
| Test No. 442E: In Vitro Skin Sensitisation | ||
| Test No. 443: Extended One-Generation Reproductive Toxicity Study | 443 Extended One-Generation Reproductive Toxicity Study | GB/T 35525-2017 Chemicals—Extended one-generation reproductive toxicity study |
| Test No. 451: Carcinogenicity Studies | 451 Carcinogenicity Studies | |
| Test No. 452: Chronic Toxicity Studies | 452 Chronic Toxicity Studies | GB/T 21759-2008 Chemicals – Test method of chronic toxicity |
| Test No. 453: Combined Chronic Toxicity/Carcinogenicity Studies | 453 Combined Chronic Toxicity/Carcinogenicity Studies | GB/T 21788-2008 Chemicals – Test method of combined chronic toxicity/carcinogenicity study |
| Test No. 455: Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists | 455 Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists | |
| Test No. 456: H295R Steroidogenesis Assay | 456 H295R Steroidogenesis Assay | |
| Test No. 457: BG1Luc Estrogen Receptor Transactivation Test Method for Identifying Estrogen Receptor Agonists and Antagonists | 457 BG1Luc Estrogen Receptor Transactivation Test Method for Identifying Estrogen Receptor Agonists and Antagonists | |
| Test No. 458: Stably Transfected Human Androgen Receptor Transcriptional Activation Assay for Detection of Androgenic Agonist and Antagonist Activity of Chemicals | ||
| Test No. 460: Fluorescein Leakage Test Method for Identifying Ocular Corrosives and Severe Irritants | 460 Fluorescein Leakage Test Method for Identifying Ocular Corrosives and Severe Irritants | |
| Test No. 467: Defined Approaches for Serious Eye Damage and Eye Irritation | ||
| Test No. 470: Mammalian Erythrocyte Pig-a Gene Mutation Assay | ||
| Test No. 471: Bacterial Reverse Mutation Test | 471,472 Bacterial Reverse Mutation Test | GB/T 21786-2008 Chemicals – Test method of bacterial reverse mutation |
| Test No. 473: In Vitro Mammalian Chromosomal Aberration Test | 473 In Vitro Mammalian Chromosomal Aberration Test | GB/T 21794-2008 Chemicals – Test method of in vitro mammalian chromosome aberration |
| Test No. 474: Mammalian Erythrocyte Micronucleus Test | 474 Mammalian Erythrocyte Micronucleus Test | GB/T 21773-2008 Chemicals – Test method of in vivo mammalian erythrocyte micronucleus |
| Test No. 475: Mammalian Bone Marrow Chromosomal Aberration Test | 475 Mammalian Bone Marrow Chromosomal Aberration Test | GB/T 21772-2008 Chemicals – Test method of mammalian bone narrow chromosome aberration |
| Test No. 476: In Vitro Mammalian Cell Gene Mutation Tests using the Hprt and xprt genes | 476 In Vitro Mammalian Cell Gene Mutation Tests | GB/T 21793-2008 Chemicals – Test method of in vitro mammalian cell gene mutation |
| Test No. 477: Genetic Toxicology: Sex-Linked Recessive Lethal Test in Drosophila melanogaster | 477 Sex-Linked Recessive Lethal Test in Drosophila melanogaster, SLRL | GB/T 27822-2011 Chemicals – Test method of sex – Linked recessive lethal in Drosophila Melanogaster |
| Test No. 478: Rodent Dominant Lethal Test | 478 Rodent Dominant Lethal Test | GB/T 21610-2008 Test method of rodent dominant lethal for chemicals |
| Test No. 479: Genetic Toxicology: In vitro Sister Chromatid Exchange Assay in Mammalian Cells | 479 In vitro Sister Chromatid Exchange Assay in Mammalian Cells | |
| Test No. 480: Genetic Toxicology: Saccharomyces cerevisiae, Gene Mutation Assay | 480 Saccharomyces cerevisiae, Gene Mutation Assay | GB/T 27831-2011 Chemicals – Genetic toxicology – Test method of Saccharomyces cerevisiae gene mutation |
| Test No. 481: Genetic Toxicology: Saacharomyces cerevisiae, Miotic Recombination Assay | 481 Saccharomyces cerevisiae, Mitotic Recombination Assay | GB/T 27832-2011 Chemicals – Genetic toxicology – Test method of Saccharomyces cerevisiae mitotic recombination |
| Test No. 482: Genetic Toxicology: DNA Damage and Repair, Unscheduled DNA Synthesis in Mammalian Cells in vitro | 482 DNA Damage and Repair, Unscheduled DNA Synthesis in Mammalian Cells in vitro | GB/T 21768-2008 Chemical – Test method of DNA damage and repair/unscheduled DNA synthesis with mammalian cells in vitro |
| Test No. 483: Mammalian Spermatogonial Chromosomal Aberration Test | 483 Mammalian Spermatogonial Chromosomal Aberration Test | GB/T 21751-2008 Chemicals – Test method of mammalian spermatogonial chromosome aberration |
| Test No. 484: Genetic Toxicology: Mouse Spot Test | 484 Mouse Spot Test | GB/T 21799-2008 Chemicals – Test method of mouse spot |
| Test No. 485: Genetic toxicology, Mouse Heritable Translocation Assay | 485 Mouse Heritable Translocation Assay | GB/T 21798-2008 Chemicals – Test method of mouse heritable translocation |
| Test No. 486: Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivo | 486 Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivo | GB/T 21767-2008 Chemical – Test method of unscheduled DNA synthesis (UDS) test with mammalian liver cells In Vivo |
| Test No. 487: In Vitro Mammalian Cell Micronucleus Test | 487 In Vitro Mammalian Cell Micronucleus Test | GB/T 28646-2012 Chemicals – Test method of in vitro mammalian cell micronucleus |
| Test No. 488: Transgenic Rodent Somatic and Germ Cell Gene Mutation Assays | 488 Transgenic Rodent Somatic and Germ Cell Gene Mutation Assays | |
| Test No. 489: In Vivo Mammalian Alkaline Comet Assay | ||
| Test No. 490: In Vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene | ||
| Test No. 491: Short Time Exposure In Vitro Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage | ||
| Test No. 492: Reconstructed human Cornea-like Epithelium (RhCE) test method for identifying chemicals not requiring classification and labelling for eye irritation or serious eye damage | ||
| Test No. 492B: Reconstructed Human Cornea-like Epithelium (RHCE) Test Method for Eye Hazard Identification | ||
| Test No. 493: Performance-Based Test Guideline for Human Recombinant Estrogen Receptor (hrER) In Vitro Assays to Detect Chemicals with ER Binding Affinity | ||
| Test No. 494: Vitrigel-Eye Irritancy Test Method for Identifying Chemicals Not Requiring Classification and Labelling for Eye Irritation or Serious Eye Damage | ||
| Test No. 495: Ros (Reactive Oxygen Species) Assay for Photoreactivity | ||
| Test No. 496: In vitro Macromolecular Test Method for Identifying Chemicals Inducing Serious Eye Damage and Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage | ||
| Guideline No. 497: Defined Approaches on Skin Sensitisation | ||
| Test No. 498: In vitro Phototoxicity – Reconstructed Human Epidermis Phototoxicity test method |